Microarray technology was used to profile miRNA expression in primary tumor and stromal tissue from paraffin embedded material of 51 patients with colorectal cancer. 26 miRNAs resulted differentially expressed with at least 2‐fold change in tumor tissue with respect to stroma (16 more expressed in the tumor and 10 more expressed in the stroma). 10/26 were confirmed as differentially expressed at qRTPCR: miR‐200c‐3p, miR‐141‐3p, miR‐200b‐3p, miR‐200a‐3p, miR‐1246, miR‐92a‐3p, miR‐194‐5p, miR‐192‐5p, miR‐3651‐5p, and miR‐574‐3p. No significant association was found between miRNA expressions and stage at diagnosis, site of primary tumor, first site of metastasis, progression‐free, or overall survival.