Analysis of genetic diversity and genome relationships of four eggplant species (Solanum melongena L) using RAPD markers

M Setyaningsih - Journal of Physics: Conference Series, 2018 - iopscience.iop.org
M Setyaningsih
Journal of Physics: Conference Series, 2018iopscience.iop.org
Solanum melongena (eggplant) is one of the diversity of the Solanum family which is grown
and widely spread in Indonesia and widely used by the community. This research explored
the genetic diversity of four local Indonesian eggplant species namely leuca, tekokak, gelatik
and kopek by using RAPD (Random Amplified Polymorphic DNA). The samples were
obtained from Agricultural Technology Assessment Institute (BPTP) Bogor, Indonesia. The
result of data observation was in the form of Solanum melongena plant's DNA profile …
Abstract
Solanum melongena (eggplant) is one of the diversity of the Solanum family which is grown and widely spread in Indonesia and widely used by the community. This research explored the genetic diversity of four local Indonesian eggplant species namely leuca, tekokak, gelatik and kopek by using RAPD (Random Amplified Polymorphic DNA). The samples were obtained from Agricultural Technology Assessment Institute (BPTP) Bogor, Indonesia. The result of data observation was in the form of Solanum melongena plant's DNA profile analyzed descriptively and quantitatively. 30 DNA bands (28 polymorphic and 2 monomorphic) were successfully scored by using four primers (OPF-01, OPF-02, OPF-03, and OPF-04). The Primers were used able to amplify all of the four eggplant samples. The result of PCR-RAPD visualization produces bands of 300-1500 bp. The result of cluster analysis showed the existence of three clusters (A, B, and C). Cluster A (coefficient of equal to 49%) consisted of a gelatik, cluster B (coefficient of 65% equilibrium) consisted of TPU (Kopek) and TK (Tekokak), and cluster C (55% equilibrium coefficient) consisted of LC (Leunca). These results indicated that the closest proximity is found in samples of TK (Tekokak) and TPU (Kopek).
iopscience.iop.org
以上显示的是最相近的搜索结果。 查看全部搜索结果