Islet transplantation is an emerging treatment for type-1 diabetes. Pancreas preservation has been identified as an area for improvement since many pancreata are exposed to> 6-8 hours of cold ischemia reducing viable islet yields. Traditionally utilized methods of preservation depend on low temperatures and specialized preservation solutions to maintain viability. Oxygenation during preservation has been identified as a potential means to extend preservation and improve isolation outcomes. The two-layer method (TLM) was developed to oxygenate pancreata during preservation. After initial excitement, since it relies on oxygen delivery by surface diffusion alone, and oxygenation of human sized pancreata in this manner is impossible; its use clinically has faded. Persufflation (PSF), gaseous oxygen perfusion, offers an alternate means of actively delivering oxygen to tissue via the vasculature. Due to the inability of small animal models to properly demonstrate oxygen limitations, and the lack of consistent availability of human pancreata a porcine model was developed.