Introduction Given the importance of β-adrenoceptor signalling in regulating cardiac
structure and function, robust protocols are required to assess potential alterations in such
regulation in murine models in vivo. Methods Echocardiography was performed in naïve and
stressed (isoprenaline; 30 μg/g/day sc for up to 14 days) mice, in the absence or presence of
acute β-adrenergic stimulation (dobutamine 0.75 μg/g, ip). Controls received saline infusion
and/or injection. Hearts were additionally analysed gravimetrically, histologically and …