The process of betalains biosynthesis by Beta vulgaris L. hairy root culture in a temporary
immersion system was studied. It was established that temporary immersion technique is
suitable for the cultivation of hairy roots. The immersion frequency had significant effect on
the biomass accumulation and the yields of betalains. The hairy root culture biosynthesized
18.8 mgg− 1 dry biomass (DB) betalains (9.6 mgg− 1 DB betacyanins and 9.2 mgg− 1 DB
betaxanthins) at immersion frequency with 15min flooding and 60min stand-by periods. The …