Biochemical characterization and homology modelling of cold-active alkophilic protease from Northwestern Himalayas and its application in detergent industry
Proteolytic enzymes are one of the largest classes of commercial enzymes used in industrial
applications especially alkophilic protease is required by detergent industry. Cold-active
alkophilic protease producing psychrotrophic bacterium was isolated from soil sediment of
Wular lake Kashmir and identified as Bacillus subtilis WLCP1. The molecular weight of
purified protease was 38 kDa, determined by SDS-PAGE. The optimal pH of the purified
protease was 10.0 and was stable at pH 7.0–11.0. The optimum temperature of the enzyme …
applications especially alkophilic protease is required by detergent industry. Cold-active
alkophilic protease producing psychrotrophic bacterium was isolated from soil sediment of
Wular lake Kashmir and identified as Bacillus subtilis WLCP1. The molecular weight of
purified protease was 38 kDa, determined by SDS-PAGE. The optimal pH of the purified
protease was 10.0 and was stable at pH 7.0–11.0. The optimum temperature of the enzyme …
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