This study aimed to analyze the amino acid profile of Kappaphycus alvarezii and Gracilaria salicornia seaweeds from Gerupuk Waters, West Nusa Tenggara (NTB). Samples were collected in January using a transect method, then dried and ground to powder to determine the amino acid content. Amino acid analysis was performed using high-performance liquid chromatography (HPLC). An amino acid standard curve was used to calculate amino acid content of each sample. The results of the analysis showed that there were 15 amino acids present in K. alvarezii seaweed, consisting of seven essential amino acids and eight non-essential amino acids. Meanwhile, G. salicornia seaweed contained 13 amino acids, consisting of seven essential amino acids and six non-essential amino acids. In K. alvarezii, the amino acid with the highest level was glutamate (13.73%), while in G. salicornia, the amino acid with the highest level was serine (13.02%). The results of the nutritional evaluation indicate that lysine is a limiting amino acid in K. alvarezii seaweed and methionine is a limiting amino acid in G. salicornia seaweed.