Surface tailoring is an existing technology in biomedical industries to improve the performance of implant materials. Surface tailoring with zirconia (ZrO₂) was performed on medical-grade stainless steel (316L SS) by electron beam physical vapor deposition (EBPVD) to enhance their properties. Monoclinic crystal phase and uniform microstructure were found in structural analysis. Superhydrophilicity nature was found on coated and bare 316L SS substrates, in the presence of lactic acid (LA) and hydrogen peroxide (H₂O₂) liquids. Pseudomonas aeruginosa (P. aeruginosa) bacterial adhesion was drastically decreased on ZrO₂ films even at 4th day of incubation. Further, superior protein adhesion as well as hemocompatible behavior were observed on ZrO₂ films. Electrochemical impedance spectroscopy (EIS) analysis revealed higher charge transfer resistance (R_ct) and corrosion protection in the presence of ZrO₂ films in artificial blood plasma (ABP) containing H₂O₂ and LA compared to bare 316L SS substrate.