A heterotrophic Pseudomonas putida CH11 was isolated from livestock farming wastewater and applied for the treatment of H₂S‐containing gas. Extensive tests including removal characteristics, metabolic products, and removal efficiencies of H₂S by P. putida CH11 were examined in batch and continuous systems. The optimum pH required to remove hydrogen sulfide was found in the range of 6–8. The maximum removal rate and the saturation constant were calculated to be V_m = 1.36 g S/day·kg dry bead and K_s = 45.9 ppm, respectively. The main metabolic product of H₂S oxidation was determined to be elemental sulfur. When P. putida CH11 was immobilized within Ca alginate, the cells exhibited high H₂S removal efficiency, in excess of 96%, at concentrations of hydrogen sulfide from 10 to 150 ppm (flow rates of 36 and 72 L/h). These results suggest that P. putida CH11 immobilized within Ca alginate has the potential to be used as a H₂S removal agent.