Rabies virus as a neurotropic agent causes rabies in humans and animals. Rabies virus transmission usually occurs through direct contact with saliva of rabid animals. However, serological and molecular tests commonly are used in diagnosing rabies but all the detection methods of rabies have some limitations. It is necessary to develop a rapid, effective, and low‐cost biosensor as an alternative tool to detect rabies virus. In this review, we studied related biosensor researches to rabies virus detection for comparing it with other detection test including serological and molecular methods. Given that very limited studies have been conducted in this field, biosensors as quick, effective, and high sensitivity tools can be used in diagnostic of rabies as an alternative tool instead of other detection methods. According to the important role of rapid detection of rabies in the control of infection and public health measures, development of a biosensor as a quick tool can be very significant in the diagnosis of rabies.