[HTML][HTML] Blastocystis in ulcerative colitis patients: Genetic diversity and analysis of laboratory findings
A Coskun, E Malatyali, H Ertabaklar, MB Yasar… - Asian Pacific journal of …, 2016 - Elsevier
Asian Pacific journal of tropical medicine, 2016•Elsevier
Abstract Objective To determine Blastocystis frequency and subtypes (ST) in ulcerative
colitis (UC) patients and analyse some laboratory findings between Blastocystis positive and
negative cases. Methods Faecal samples from 150 UC patients in Adnan Menderes
University, Training and Research Hospital were examined by direct microscopy and
cultivated in Jones medium. Blastocystis positive cultures were subjected to DNA isolation
and subtypes were identified by sequencing of barcode region. A retrospective analysis was …
colitis (UC) patients and analyse some laboratory findings between Blastocystis positive and
negative cases. Methods Faecal samples from 150 UC patients in Adnan Menderes
University, Training and Research Hospital were examined by direct microscopy and
cultivated in Jones medium. Blastocystis positive cultures were subjected to DNA isolation
and subtypes were identified by sequencing of barcode region. A retrospective analysis was …
Objective
To determine Blastocystis frequency and subtypes (ST) in ulcerative colitis (UC) patients and analyse some laboratory findings between Blastocystis positive and negative cases.
Methods
Faecal samples from 150 UC patients in Adnan Menderes University, Training and Research Hospital were examined by direct microscopy and cultivated in Jones medium. Blastocystis positive cultures were subjected to DNA isolation and subtypes were identified by sequencing of barcode region. A retrospective analysis was conducted on C reactive protein (CRP), leucocyte counts (WBC), neutrophil counts, and sedimentation rates.
Results
The overall positive rate of Blastocystis was 8% (12 patients) and the most abundant subtype was ST3 (eight isolates, 66.7%), followed by ST1, ST2 and ST7. Laboratory findings between Blastocystis infected and non-infected UC patients were not significantly different. Blastocystis frequency was 3.8% among the patients in active stage, while it was 11.8% among the patients in remission stage.
Conclusions
The present study confirms previous findings that have indicated the predominance of Blastocystis ST3 in humans and contributes additional evidence that suggests the low colonisation of Blastocystis infection in ulcerative colitis patients during active stage.
Elsevier
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