COX-2-dependent and-independent biosynthesis of dihydroxy-arachidonic acids in activated human leukocytes
Biosynthesis of 5, 15-dihydroxyeicosatetraenoic acid (5, 15-diHETE) in leukocytes involves
consecutive oxygenation of arachidonic acid by 5-lipoxygenase (LOX) and 15-LOX in either
order. Here, we analyzed the contribution of cyclooxygenase (COX)-2 to the biosynthesis of
5, 15-diHETE and 5, 11-diHETE in isolated human leukocytes activated with
lipopolysaccharide and calcium ionophore A23187. Transformation of arachidonic acid was
initiated by 5-LOX providing 5S-HETE as a substrate for COX-2 forming 5S, 15S-diHETE …
consecutive oxygenation of arachidonic acid by 5-lipoxygenase (LOX) and 15-LOX in either
order. Here, we analyzed the contribution of cyclooxygenase (COX)-2 to the biosynthesis of
5, 15-diHETE and 5, 11-diHETE in isolated human leukocytes activated with
lipopolysaccharide and calcium ionophore A23187. Transformation of arachidonic acid was
initiated by 5-LOX providing 5S-HETE as a substrate for COX-2 forming 5S, 15S-diHETE …