[HTML][HTML] CRISPR/Cas9-mediated adenine base editing in rice genome
LI Hao, QIN Ruiying, LIU Xiaoshuang, L Shengxiang… - Rice Science, 2019 - Elsevier
LI Hao, QIN Ruiying, LIU Xiaoshuang, L Shengxiang, XU Rongfang, Y Jianbo…
Rice Science, 2019•ElsevierPrecise base editing is highly desired in plant functional genomic research and crop
molecular breeding. In this study, we constructed a rice-codon optimized adenine base
editor (ABE)-nCas9 tool that induced targeted A· T to G· C point mutation of a key single
nucleotide polymorphism site in an important agricultural gene. Combined with the modified
single-guide RNA variant, our plant ABE tool can efficiently achieve adenine base editing in
the rice genome.
molecular breeding. In this study, we constructed a rice-codon optimized adenine base
editor (ABE)-nCas9 tool that induced targeted A· T to G· C point mutation of a key single
nucleotide polymorphism site in an important agricultural gene. Combined with the modified
single-guide RNA variant, our plant ABE tool can efficiently achieve adenine base editing in
the rice genome.
Summary
Precise base editing is highly desired in plant functional genomic research and crop molecular breeding. In this study, we constructed a rice-codon optimized adenine base editor (ABE)-nCas9 tool that induced targeted A·T to G·C point mutation of a key single nucleotide polymorphism site in an important agricultural gene. Combined with the modified single-guide RNA variant, our plant ABE tool can efficiently achieve adenine base editing in the rice genome.
Elsevier
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