CUC2 as an early marker for regeneration competence in Arabidopsis root explants
Abstract CUP SHAPED COTELYDON 2 (CUC2) was tested as a marker for shoot induction
to monitor and facilitate the optimization of in vitro regeneration of Arabidopsis thaliana. The
expression of a pCUC2:: 3XVENUS-N7 fluorescent marker allowed the observation of early
steps in the initiation and development of shoots on root explants. The explants were first
incubated on an auxin-rich callus induction medium (CIM) and then transferred to a cytokinin-
rich shoot induction medium (SIM). CUC2-expression occurred prior to visible shoot …
to monitor and facilitate the optimization of in vitro regeneration of Arabidopsis thaliana. The
expression of a pCUC2:: 3XVENUS-N7 fluorescent marker allowed the observation of early
steps in the initiation and development of shoots on root explants. The explants were first
incubated on an auxin-rich callus induction medium (CIM) and then transferred to a cytokinin-
rich shoot induction medium (SIM). CUC2-expression occurred prior to visible shoot …
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