Calorimetric and fluorescence techniques were used to characterize the binding of
aminoglycosides-neomycin, paromomycin, and ribostamycin, with 5′-dA12-x-dT12-x-dT12-
3′ intramolecular DNA triplex (x= hexaethylene glycol) and poly (dA)· 2poly (dT) triplex. Our
results demonstrate the following features:(1) UV thermal analysis reveals that the Tm for
triplex decreases with increasing pH value in the presence of neomycin, while the Tm for the
duplex remains unchanged.(2) The binding affinity of neomycin decreases with increased …