Cell refractive index tomography by digital holographic microscopy
F Charrière, A Marian, F Montfort, J Kuehn, T Colomb… - Optics letters, 2006 - opg.optica.org
Optics letters, 2006•opg.optica.org
For what we believe to be the first time, digital holographic microscopy is applied to perform
optical diffraction tomography of a pollen grain. Transmission phase images with nanometric
axial accuracy are numerically reconstructed from holograms acquired for different
orientations of the rotating sample; then the three-dimensional refractive index spatial
distribution is computed by inverse radon transform. A precision of 0.01 for the refractive
index estimation and a spatial resolution in the micrometer range are demonstrated.
optical diffraction tomography of a pollen grain. Transmission phase images with nanometric
axial accuracy are numerically reconstructed from holograms acquired for different
orientations of the rotating sample; then the three-dimensional refractive index spatial
distribution is computed by inverse radon transform. A precision of 0.01 for the refractive
index estimation and a spatial resolution in the micrometer range are demonstrated.
For what we believe to be the first time, digital holographic microscopy is applied to perform optical diffraction tomography of a pollen grain. Transmission phase images with nanometric axial accuracy are numerically reconstructed from holograms acquired for different orientations of the rotating sample; then the three-dimensional refractive index spatial distribution is computed by inverse radon transform. A precision of 0.01 for the refractive index estimation and a spatial resolution in the micrometer range are demonstrated.
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