[PDF][PDF] Characterization of an endophytic bacterial community associated with Eucalyptus spp

WL Araújo, W Maccheroni, JL Azevedo - Genetics and Molecular …, 2009 - Citeseer
Genetics and Molecular Research, 2009Citeseer
Endophytic bacteria were isolated from stems of Eucalyptus spp (Eucalyptus citriodora, E.
grandis, E. urophylla, E. camaldulensis, E. torelliana, E. pellita, and a hybrid of E. grandis
and E. urophylla) cultivated at two sites; they were characterized by RAPD and amplified
rDNA restriction analysis (ARDRA). Endophytic bacteria were more frequently isolated from
E. grandis and E. pellita. The 76 isolates were identified by 16S rDNA sequencing as
Erwinia/Pantoea (45%), Agrobacterium sp (21%), Curtobacterium sp (9%), Brevibacillus sp …
Abstract
Endophytic bacteria were isolated from stems of Eucalyptus spp (Eucalyptus citriodora, E. grandis, E. urophylla, E. camaldulensis, E. torelliana, E. pellita, and a hybrid of E. grandis and E. urophylla) cultivated at two sites; they were characterized by RAPD and amplified rDNA restriction analysis (ARDRA). Endophytic bacteria were more frequently isolated from E. grandis and E. pellita. The 76 isolates were identified by 16S rDNA sequencing as Erwinia/Pantoea (45%), Agrobacterium sp (21%), Curtobacterium sp (9%), Brevibacillus sp (8%), Pseudomonas sp (8%), Acinetobacter sp (4%), Burkholderia cepacia (2.6%), and Lactococcus lactis (2.6%). Genetic characterization of these endophytic bacteria isolates showed at least eight ARDRA haplotypes. The genetic diversity of 32 Erwinia/Pantoea and 16 Agrobacterium sp isolates was assessed with the RAPD technique. There was a high level of genetic polymorphism among all the isolates and there was positive correla-
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