Wheat cultivars with wide introgression have strongly impacted global wheat production. Aegilops geniculata (M^gU^g) is an important wild relative with several useful traits that can be exploited for wheat improvement. Screening of Ae. geniculata addition lines indicated a negative effect of 1U^g and the positive effect of 1M^g chromosome on wheat dough strength. Negative effect of 1U^g is probably associated with variation in number and position of the tripeptide repeat motif in the high molecular weight glutenin (HMW-G) gene. To utilize the positive potential of 1M^g chromosome, three disomic substitution lines (DSLs) 1M^g(1A), 1M^g(1B) and 1M^g(1D) were created. These lines were characterized for morphological, cytogenetic properties and biochemical signatures using FISH, 1D-, 2D-PAGE and RP-HPLC. Contribution of wheat 1A, 1B and 1D chromosomes towards dough mixing and baking parameters, chapatti quality, Fe/Zn content and glume color were identified. Observed order of variation in the dough mixing and baking parameters {1M^g(1D) ≤wheat ≤1M^g(1B) ≤1M^g(1A)} indicated that chromosome specific introgression is desirable for best utilization of wild species’ potential.