Angiogenesis is now well known for being involved in tumor progression, aggressiveness,
emergence of metastases, and also resistance to cancer therapies. In this study, to better
mimic tumor angiogenesis encountered in vivo, we used 3D culture of osteosarcoma cells
(MG-63) that we deposited on 2D endothelial cells (HUVEC) grown in monolayer. We report
that endothelial cells combined with tumor cells were able to form a well-organized network,
and that tubule-like structures corresponding to new vessels infiltrate tumor spheroids …

Background: The number of patients suffering from cancers worldwide is increasing, and
one of the most challenging issues in oncology continues to be the problem of developing
active drugs economically and in a timely manner. Considering the high cost and time-
consuming nature of the clinical development of oncology drugs, better pre-clinical platforms
for drug screening are urgently required. So, there is a need for high-throughput drug
screening platforms to mimic the in vivo microenvironment. Angiogenesis is now well known …