Comparison of Different Metal Film Thicknesses of COC-Substrate Polymerase Chain Reaction Chips With Single-Side and Double-Side Heaters
YC Chung, LW Lai, LJ Yang… - ASME …, 2009 - asmedigitalcollection.asme.org
YC Chung, LW Lai, LJ Yang, WJ Liao
ASME International Mechanical Engineering Congress and …, 2009•asmedigitalcollection.asme.orgWe fabricated PCR chips of different metal film thicknesses. The PCR machine with/without
top heater and the single-side/double-side heater PCR chips were compared. The substrate
was transparent COC (Cyclic Olefin Copolymers), and the gold film was fabricated on the
substrate by evaporation without any adhesive layer. Then the results after polymerase
chain reaction for the DNA of Escherichia coli TG-1 cell were obtained. The concentrations
of amplifying 225 bps DNA were 0.78–0.82 ng/μl (64% of the result of PCR machine).
top heater and the single-side/double-side heater PCR chips were compared. The substrate
was transparent COC (Cyclic Olefin Copolymers), and the gold film was fabricated on the
substrate by evaporation without any adhesive layer. Then the results after polymerase
chain reaction for the DNA of Escherichia coli TG-1 cell were obtained. The concentrations
of amplifying 225 bps DNA were 0.78–0.82 ng/μl (64% of the result of PCR machine).
We fabricated PCR chips of different metal film thicknesses. The PCR machine with/without top heater and the single-side/double-side heater PCR chips were compared. The substrate was transparent COC (Cyclic Olefin Copolymers), and the gold film was fabricated on the substrate by evaporation without any adhesive layer. Then the results after polymerase chain reaction for the DNA of Escherichia coli TG-1 cell were obtained. The concentrations of amplifying 225 bps DNA were 0.78–0.82 ng/μl (64% of the result of PCR machine).
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