The paper deals with research of air pollution in two different locations on the Moravian-Silesian Region, Czech Republic. These are the sites Ostrava-Radvanice, which is located in the area affected by the industry and Ostravice in the mountains (without significant effect of the industry). The dust particles collected at these locations were subjected to a wide range of analyses. The mass concentration, the mass-size distribution, mineralogical composition, the concentration of PAHs (polycyclic aromatic hydrocarbons), and the concentrations of selected metals (Cd, Pb, Zn, Fe, Mn, As, Ni, Co, and Cr) were observed at the particulate matter.