Comprehensive characterization of single-cell full-length isoforms in human and mouse with long-read sequencing
A modified Chromium 10x droplet-based protocol that subsamples cells for both short-read
and long-read (nanopore) sequencing together with a new computational pipeline
(FLAMES) is developed to enable isoform discovery, splicing analysis, and mutation
detection in single cells. We identify thousands of unannotated isoforms and find conserved
functional modules that are enriched for alternative transcript usage in different cell types
and species, including ribosome biogenesis and mRNA splicing. Analysis at the transcript …
and long-read (nanopore) sequencing together with a new computational pipeline
(FLAMES) is developed to enable isoform discovery, splicing analysis, and mutation
detection in single cells. We identify thousands of unannotated isoforms and find conserved
functional modules that are enriched for alternative transcript usage in different cell types
and species, including ribosome biogenesis and mRNA splicing. Analysis at the transcript …
[引用][C] Comprehensive characterization of single cell full-length isoforms in human and mouse with long-read sequencing. preprint
L Tian, JS Jabbari, R Thijssen, Q Gouil… - Genomics, August, 2020
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