Publisher Summary The early embryo of Caenorhabditis elegans is one of the most powerful
model systems to study cell division. This chapter develops a correlative light and electron
microscopic approach to stage early C. elegans embryos prior to high-pressure freezing and
electron tomography. Cellulose microcapillaries are used to contain the embryos in short
transparent tubes. These permit the viewing of early mitotic events under a light microscope.
Next step is to stage selected embryos by light microscopy (LM), to immobilize these staged …