The central catalyst in eukaryotic ATP-dependent homologous recombination consists of
RAD51 proteins, polymerized around single-stranded DNA. This nucleoprotein filament
recognizes and invades a homologous duplex DNA segment,. After strand exchange, the
nucleoprotein filament should disassemble so that the recombination process can be
completed. The molecular mechanism of RAD51 filament disassembly is poorly understood.
Here we show, by combining optical tweezers with single-molecule fluorescence …

The central catalyst in eukaryotic ATP-dependent homologous recombination consists of
RAD51 proteins, polymerized around single-stranded DNA. This nucleoprotein filament
recognizes a homologous duplex DNA segment and invades it. After strand exchange, the
nucleoprotein filament should disassemble in order for the recombination process to
complete. The molecular mechanism of RAD51 filament disassembly is poorly understood.
Here, we have combined optical tweezers with single-molecule fluorescence microscopy …