The intracellular environment in which biological reactions occur is crowded with
macromolecules and subdivided into microenvironments that differ in both physical
properties and chemical composition. The work described here combines experimental and
computational model systems to help understand the consequences of this heterogeneous
reaction media on the outcome of coupled enzyme reactions. Our experimental model
system for solution heterogeneity is a biphasic polyethylene glycol (PEG)/sodium citrate …