Cyanobacteria are the ideal organisms for the production of a wide range of bioproducts as
they can convert CO2 directly into the desired end product using solar energy. Unfortunately,
the engineering of cyanobacteria to create efficient cell factories has been impaired by the
cumbersome genetic tools that are currently available for these organisms; especially when
trying to accumulate multiple modifications. We sought to construct an efficient and precise
tool for generating numerous markerless modifications in cyanobacteria using CRISPR …