Reports on the vitrification of somatic cells are scarce. Here, we show that Leydig cells
(murine cell line TM3) could be successfully vitrified by both open vitrification [plastic straw
(PS) and plastic vials (PV)] and closed ultravitrification [microdrop (MD) and solid surface
vitrification (SSV)], after protocol optimization. However, open ultravitrification resulted in
better post-warming viability than closed systems of vitrification with highest success
obtained in modified SSV (84.8±1.86%; p< 0.05). Leydig cells vitrified-warmed by modified …