The early detection of bacterial attachment is very crucial in the prevention of biofilm growth because fully established biofilms are extremely resistant to chemical or physical treatments. The cyclic voltammentry was employed to differentially monitor bacterial attachment and biofilm formation on electrodes in this study. In addition, the mathematical estimation of the surface coverage from the cyclic voltammogram was tried and it was found that the estimation had a linear relationship with the actual coverage of the electrode, validating the potential of cyclic voltammetry for getting the quantitative information about the degree of the bacterial attachment on the surface.