[PDF][PDF] Cytological aberrations in M2 morphological mutants of Linum usitatissimum (L.) induced by physical and chemical mutagens
At present, the significant worry of the plant breeding researchers is to enhance the yield of
various crops including linseed worldwide. By exploring the potential of chemical mutagens,
ionizing radiation and hybridization technique, scientists are more concerned to improve
quality and quantity both. The seeds of linseed plant were treated with different doses of
gamma-rays (50Gy, 100Gy, 150Gy, 200Gy) and sodium azide (0.1% SA, 0.2% SA, 0.3% SA
0.4% SA) which induces various morphological putative mutants in M2 generation such as …
various crops including linseed worldwide. By exploring the potential of chemical mutagens,
ionizing radiation and hybridization technique, scientists are more concerned to improve
quality and quantity both. The seeds of linseed plant were treated with different doses of
gamma-rays (50Gy, 100Gy, 150Gy, 200Gy) and sodium azide (0.1% SA, 0.2% SA, 0.3% SA
0.4% SA) which induces various morphological putative mutants in M2 generation such as …
Abstract
At present, the significant worry of the plant breeding researchers is to enhance the yield of various crops including linseed worldwide. By exploring the potential of chemical mutagens, ionizing radiation and hybridization technique, scientists are more concerned to improve quality and quantity both. The seeds of linseed plant were treated with different doses of gamma-rays (50Gy, 100Gy, 150Gy, 200Gy) and sodium azide (0.1% SA, 0.2% SA, 0.3% SA 0.4% SA) which induces various morphological putative mutants in M2 generation such as bushy mutant (0.2% SA), narrow leaf mutant (100 Gy), and broad stem mutant (150Gy). The doses given were found appropriate to achieve the objective for increasing the productivity of linseed, focusing less biological damage with more variation in quantitative traits. In moderate and higher doses of chemical and physical mutagens, various morphological variants affecting almost all parts of the plant were observed. The cytological analysis with the manifestation of chromosomal abnormalities includes precocious movement of chromosome, unequal separation of the chromosome, laggard and chromosome bridge. Induction of morphological alterations and chromosomal aberrations were found to be higher in gamma rays treated populations than sodium azide comparatively. However the molecular mechanism responsible for the genetic modifications in these putative mutants needs to also be explored through other supporting experiments. Accelerating the rate of desirable high-yield mutants was shown to be economically important. From future perspective it has been correlated that the segregation of selected mutants will definitely contribute to the improved linseed genotype and these mutants may also be used as valuable breeding stock.
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