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Check for updates Chapter 7 Optimized Protocol for the Regulation of DNA Methylation and Gene Expression Using Modified dCas9-SunTag Platforms Sumiyo Morita …

S Morita - Epigenome Editing: Methods and Protocols - books.google.com
7 天前 - … In this chapter, we present an exemplary protocol for DNA methylation editing …
DNA methylation editing in cultured cells and quantitative measurements of the DNA methylation …
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Development of Locus-Directed Editing of the Epigenome from Basic Mechanistic Engineering to First Clinical Applications

MG Rots, A Jeltsch - Epigenome Editing: Methods and Protocols, 2024 - Springer
8 天前 - … developed targeted DNA methylation, a method in which a DNA methyltransferase
is … binding sites resulting in the introduction of DNA methylation at these sites. In a seminal …
相关文章 所有 4 个版本

Whole-Genome Bisulfite Sequencing Protocol for the Analysis of Genome-Wide DNA Methylation and Hydroxymethylation Patterns at Single-Nucleotide Resolution

D Derbala, A Garnier, E Bonnet, JF Deleuze… - … : Methods and Protocols, 2024 - Springer
8 天前 - … Here, we provide a detailed protocol based on a commercial kit for the preparation
of … genome analysis of DNA methylation and/or hydroxymethylation. The protocol is based on …
相关文章 所有 3 个版本

Reader-Effectors as Actuators of Epigenome Editing

SH Kim, KA Haynes - Epigenome Editing: Methods and Protocols, 2024 - Springer
8 天前 - … For instance, exactly how will an altered chromatin modification such as DNA
methylation or histone acetylation, methylation, etc. be interpreted by the transcriptional and …
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An Overview of Global, Local, and Base-Resolution Methods for the Detection of 5-Hydroxymethylcytosine in Genomic DNA

N Erlitzki, RM Kohli - Epigenome Editing: Methods and Protocols, 2024 - Springer
8 天前 - … For example, while the majority of DNA methylation in the mammalian genome
occurs at CpG sites, ~25% of 5mC in embryonic stem cells can be found at non-CG (ie, CHG or …
相关文章 所有 4 个版本

Protocol for Allele-Specific Epigenome Editing Using CRISPR/dCas9

N Rajaram, P Bashtrykov, A Jeltsch - … Editing: Methods and Protocols, 2024 - Springer
8 天前 - … of DNA methylation is required exclusively in a single allele. Here, we describe a
basic protocol for … editing systems using allele-specific DNA methylation at the NARF gene in …
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Protocol for DNA Methylation Editing of Imprinted Loci and Assessment of the Effects

Y Sekita, T Kimura - Epigenome Editing: Methods and Protocols, 2024 - Springer
8 天前 - … experimental protocol to conduct DNA methylation editing … present an exemplary
protocol for DNA methylation editing … We introduce the methods for DNA methylation editing in …
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Amplicon-Based Bisulfite Conversion-NGS DNA Methylation Analysis Protocol

C Albrecht, P Bashtrykov, A Jeltsch - … Editing: Methods and Protocols, 2024 - Springer
8 天前 - … target regions from bisulfite-converted DNA, followed by a … approach to extract
DNA methylation levels of the target … of DNA methylation patterns at defined genomic regions. …
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Optimized Protocol for the Regulation of DNA Methylation and Gene Expression Using Modified dCas9-SunTag Platforms

S Morita, T Horii, I Hatada - Epigenome Editing: Methods and Protocols, 2024 - Springer
8 天前 - … protocol for the application of a modified dCas9-SunTag system, which increased
the efficiency of targeted demethylation and gene activation at specific DNA … DNA methylation …
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Protocol for Efficient Generation of Chimeric Antigen Receptor T Cells with Multiplexed Gene Silencing by Epigenome Editing

MSR Azcona, C Mussolino - Epigenome Editing: Methods and Protocols, 2024 - Springer
8 天前 - … To corroborate the association of the silencing with the deposition of DNA methylation
… antigen stimulation assay and assess DNA methylation in a region of approximately 400 …
相关文章 所有 2 个版本