For the fungal pathogen Candida albicans, genetic overexpression readily occurs via a
diversity of genomic alterations, such as aneuploidy and gain-of-function mutations, with
important consequences for host adaptation, virulence, and evolution of antifungal drug
resistance. Given the important role of overexpression on C. albicans biology, it is critical to
develop and harness tools that enable the analysis of genes expressed at high levels in the
fungal cell. Here, we describe the development, optimization, and application of a novel …