This study examined the ability of epidermal growth factor (EGF) to improve the
developmental competence of pig oocytes matured in a protein-free (PF) in vitro maturation
(IVM) system. Oocyte maturation was done in one of three media: 1. PF-TCM: tissue culture
medium (TCM) 199+ 0.1% polyvinylalcohol (PVA); 2. PF-TCM+ EGF: PF-TCM+ 10 ng/ml
EGF; and 3.+ ve CONT: North Carolina State University (NCSU) 23 medium+ 10% porcine
follicular fluid. All media contained 0.57 mM cysteine. Hormonal supplements, 0.5 μg/mL LH …