Development of a SYBR Green quantitative PCR assay for detection of Lates calcarifer herpesvirus (LCHV) in farmed barramundi
W Meemetta, JA Domingos, HT Dong… - Journal of Virological …, 2020 - Elsevier
Lates calcarifer herpes virus (LCHV) is a novel virus of farmed barramundi in Southeast
Asia. However, a rapid detection method is yet to be available for LCHV. This study,
therefore, aimed to develop a rapid quantitative PCR (qPCR) detection method for LCHV
and made it timely available to public for disease diagnostics and surveillance in
barramundi farming countries. A newly designed primer set targeting a 93-bp fragment of the
LCHV putative major envelope protein encoding gene (MEP) was used for developing and …
Asia. However, a rapid detection method is yet to be available for LCHV. This study,
therefore, aimed to develop a rapid quantitative PCR (qPCR) detection method for LCHV
and made it timely available to public for disease diagnostics and surveillance in
barramundi farming countries. A newly designed primer set targeting a 93-bp fragment of the
LCHV putative major envelope protein encoding gene (MEP) was used for developing and …
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