We used 3D cell printing to emulate an airway coupled with a naturally-derived blood vessel
network in vitro. Decellularized extracellular matrix bioink derived from porcine tracheal
mucosa (tmdECM) was used to encapsulate and print endothelial cells and fibroblasts within
a designated polycarprolactone (PCL) frame. Providing a niche that emulates conditions in
vivo, tmdECM gradually drives endothelial re-orientation, which leads to the formation of a
lumen and blood vessel network. A fully-differentiated in vitro airway model was assembled …