Diverse expression of selected cytokines and proteinases in synovial fluid obtained from osteoarthritic and healthy human knee joints
M Sauerschnig, J Stolberg-Stolberg, A Schulze… - European Journal of …, 2014 - Springer
M Sauerschnig, J Stolberg-Stolberg, A Schulze, GM Salzmann, C Perka, CJ Dynybil
European Journal of Medical Research, 2014•SpringerBackground Osteoarthritis (OA) is defined by signs and symptoms of inflammation within the
affected joint. The aim of this study is to determine the mRNA expression levels of selected
cytokines and matrix-metalloproteinases of cells found in synovial fluid (SF) obtained from
osteoarthritic knee joints compared to healthy controls. Methods SF was obtained from 40
patients undergoing total knee arthroplasty due to evident OA and from 10 healthy controls.
Expression of TNF-α, IL-1β, MMP-1 and MMP-3 was assayed among both groups by …
affected joint. The aim of this study is to determine the mRNA expression levels of selected
cytokines and matrix-metalloproteinases of cells found in synovial fluid (SF) obtained from
osteoarthritic knee joints compared to healthy controls. Methods SF was obtained from 40
patients undergoing total knee arthroplasty due to evident OA and from 10 healthy controls.
Expression of TNF-α, IL-1β, MMP-1 and MMP-3 was assayed among both groups by …
Background
Osteoarthritis (OA) is defined by signs and symptoms of inflammation within the affected joint. The aim of this study is to determine the mRNA expression levels of selected cytokines and matrix-metalloproteinases of cells found in synovial fluid (SF) obtained from osteoarthritic knee joints compared to healthy controls.
Methods
SF was obtained from 40 patients undergoing total knee arthroplasty due to evident OA and from 10 healthy controls. Expression of TNF-α, IL-1β, MMP-1 and MMP-3 was assayed among both groups by performing qPCR. Patients were configured concerning age, gender and BMI.
Results
IL-1β, MMP-1 and MMP-3 showed significantly higher expression among the OA group compared to control (P < 0.001). Strong correlation appeared between expression of MMP-1 and MMP-3 among OA patients (r = 0.856); no correlation was found between age, gender or BMI and cytokine/proteinase expression. Expression of IL-1β, MMP-1 and MMP-3 within SF was elevated in OA-patients.
Conclusion
Consequently, cells within SF expressing cytokines and proteinases may play a relevant role in the progression of joint destruction. Considering the fact that SF in an OA joint comprises abnormal amounts of detrimental bioactive proteins, temporary clearance, dilution or suppression/modulation by means of lavage or disease-modifying medication may be promising to constitute interim relief or even postpone disease progression due to decreased inflammatory and/or degrading activity within the articular environment.
Springer
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