We performed a prospective cohort study in 197 pregnant women. Peripheral blood was
collected between 5 and 16 weeks of gestation. Intracellular cytokine analysis and
immunophenotype were performed by flow-cytometry. Serum levels of cytokines and
chemokines were analyzed by multiplex assay. 86 patients were eligible for the analysis and
10.5%(n= 9) developed preeclampsia. Patients with preeclampsia had significantly higher
percentage of CD3+ CD4+ TNFα+ T helper (Th) 1 cells (45.4±10.3 vs 37.1±8.5, P= 0.032) …