We investigated the effects of different O₂ concentrations (air or 2 kPa O₂) and temperatures (5 °C or 20 °C) on the respiration rate, betacyanin content and VC content to provide basic information on the most optimal conditions for practical storage and transportation of red amaranth. The respiration rates were obviously reduced by low temperatures and low O₂ treatments. However, an increase in the CO₂ production was detected in red amaranth stored at 20 °C-2 kPa O₂ after 30 h. The total betacyanin content changed slightly after storage for 3 days, and there was no significant difference among storage treatments. The VC contents of all the treatments were greatly reduced (more than 50%) after storage. The VC content of red amaranth stored under low temperature either with or without low O₂ was not different from the control. Nevertheless, low O₂ (2 kPa) at 20 °C significantly slowed the loss of VC. Our results mainly suggested that the O₂ level is one of the key factors affecting the quality of red amaranth during storage.