The main aim of this study is to examine the different film preparation methods (Hot air oven method, Microwave oven method, and UV irradiation method) for development of Bio-thermoplastic films from agro waste polysaccharides. The rheological properties were also studied for different combinations of tamarind seed starch (T), okra mucilage polysaccharide (OK), Residual rice bran starch (R) and sugarcane bagasse cellulose (C). Increase in concentration of okra mucilage polysaccharide and sugarcane bagasse cellulose found to highly influence the rheological viscous properties of tamarind starch solution. Increase in concentration of okra mucilage (above 1.5%) in tamarind film matrix solution significantly increased the elongation properties of starch films. Increase in concentration of cellulose (above 1%) in tamarind film matrix solution significantly increased the tensile strength of starch films. The microwave oven method was found to be the quickest method (~10 to 20 min) for biopolymer film preparation. SEM and AFM analysis revealed that UV – irradiation method produces stable bio-thermoplastic films with low surface roughness and high barrier properties. The interaction of starch molecules with other natural polysaccharide produces stable thermoplastic biopolymer films, that can be developed under different processing conditions to eradicate environmental pollution caused by petrochemical plastics.