In two years study on the response of black gram (Vigna mungo L. Hepper) Cv JU 2, the optimum level of phosphorus through different sources was determined with or without application of PSB [Phosphorus solubilizing bacteria]. Significantly highest seed yield of 651 kg/ha was recorded due to application of 40 Kg P2O5/ha through DAP with PSB. The increase in seed yield was attributed mainly due to increase in nodulation, plant height, branches per plant, leaves per plant and pods per plant. A net return of Rs. 2624/- per hectare was also recorded highest in this treatment. It is therefore, recommended for general adoption in medium black soils of Madhya Pradesh.