The purpose of this study to demonstrate the effects of lysine supplement on small intestinal morphology of broilers chickens. In starter period control group received diet containing 1.2% lysine, Treatment 1 [T1] and Treatment 2 [T2] groups received diet containing 1.3% and 1.4% lysine, respectively. Intestine villous morphology examination was done by analyzing of villi's height, width, depth of crypts and the type of villous. The finding shown that as diet lysine increased, the villous height had a significant increase compared to the control group. In the starter period, the growth of intestine villous width which depends on lysine density had significant increase (P< 0.05) compared to control group. As T group lysine increased (P< 0.05) compared to 2 control Group, Libercuhn crypt depth had a significant increase (P< 0.05). A lot of intestine villous were in a kind of leaf and tongue and also a little percent were bridge villi, Finger, Ridge and convoluted villi. In duodenum, different levels of lysine on the tongue and leaf villi had not significant effect. In the starter period, lysine increase in T and T lead to significant decrease (P< 0.05) of leaf villous compared to control but has caused