Salmonella typhi, Shigella dysentariae and Esherichia coli 0157: H7 was carried out using agar diffusion method. The palm wine used had growth inhibitory effect on all the test organisms with diameter of zones of inhibition ranging from 2.20 mm to 28.20 mm. Palm wine subjected to fermentation for 168hours (7 days) exerted the highest growth inhibitory effect on all the test bacteria. The inhibition mediated by this palm wine was superior to that of some conventional antibiotics used such as tetracycline, ampiclox and ampicillin. It is conceivable therefore that palm wine subjected to natural fermentation or freshly tapped could be used to treat diarrhoea caused by these organisms.