Spices are used as additives to flavour, colour or preserve food from ancient time in India. These are pungent or aromatic substances obtained from by dried seeds, fruits, roots, bark or leaves. Spices are the potential source of natural products and naturally derived compounds. These compounds show the antioxidant, antimicrobial properties. The aim of this study is to evaluate the antimicrobial properties of Brassica nigra. In this study, antimicrobial activities of 70% ethanolic seed extracts of Brassica nigra were evaluated against different bacterial strains (E. coli, Pseudomonas aeruginosa, Candida albicans) by agar well diffusion method & MIC determination by broth dilution method. The extract showed a broad spectrum of antimicrobial activities by inhibiting the growth of respective microorganism in Agar well diffusion assay. The present study supports the immense medicinal properties of Brassica nigra. It may be helpful for future researches in area of new drug development of herbal origins.