Efficient recombinant production of prodigiosin in Pseudomonas putida

A Domröse, AS Klein, J Hage-Hülsmann… - Frontiers in …, 2015 - frontiersin.org
… demonstrate efficient prodigiosin production in the heterologous host Pseudomonas putida.
… In summary, we report here effective biosynthetic prodigiosin production by expression of …

Stepwise genetic engineering of Pseudomonas putida enables robust heterologous production of prodigiosin and glidobactin A

TB Cook, TB Jacobson, MV Venkataraman… - Metabolic …, 2021 - Elsevier
… titers, such as GC content of the BGC, translational efficiency, and specific activity of PKSs
and NRPSs, … The integration vector carries out a single-crossover recombination with the P. …

New Prodigiosin Derivatives Obtained by Mutasynthesis in Pseudomonas putida

AS Klein, A Domröse, P Bongen… - ACS synthetic …, 2017 - ACS Publications
… ) deficient, but still MBC (5) producing mutant of this safe recombinant production strain
presents a fast and cost efficient alternative to chemical synthesis of prodigiosin (1a) derivatives. …

Pseudomonas putida rDNA is a favored site for the expression of biosynthetic genes

A Domröse, J Hage-Hülsmann, S Thies, R Weihmann… - Scientific reports, 2019 - nature.com
… , are often naturally produced in only low amounts, their efficient … We could further demonstrate
that prodigiosin production was … of growth and the recombinant prodigiosin pathway in P. …

[HTML][HTML] Rapid generation of recombinant Pseudomonas putida secondary metabolite producers using yTREX

A Domröse, R Weihmann, S Thies, KE Jaeger… - Synthetic and systems …, 2017 - Elsevier
putida strains producing prodigiosin were grown in TB medium and used to inoculate …
As demonstrated in this study, effective gene cluster expression can be achieved in P. …

Engineering of natural product biosynthesis in Pseudomonas putida

A Loeschcke, S Thies - Current opinion in biotechnology, 2020 - Elsevier
… bacterium Pseudomonas putida represents an effective production host. … production strains
or technology platforms can be developed as shown for recombinant prodigiosin production. …

A comprehensive review on recent trends in production, purification, and applications of prodigiosin

T Paul, TK Bandyopadhyay, A Mondal… - Biomass Conversion …, 2020 - Springer
Prodigiosin isolated from bacteria and other compounds synthetically derived from this red
pigment are very much effective as … of prodigiosin in a recombinant Pseudomonas putida

Markerless gene knockout and integration to express heterologous biosynthetic gene clusters in Pseudomonas putida

KR Choi, JS Cho, IJ Cho, D Park, SY Lee - Metabolic engineering, 2018 - Elsevier
… Subsequently, we develop an efficient system for markerless integration of heterologous …
putida strains cured of the RecET vector, the recombinant strains harboring the RecET vectors …

Prodigiosin production and recovery from Serratia marcescens: process development and cost–benefit analysis

T Paul, A Mondal, TK Bandyopadhyay… - Biomass Conversion and …, 2024 - Springer
Prodigiosin was found to be very much effective in the inhibition of tumor cell and induction
of … for construction of recombinant microorganism which is able to produce higher amount of …

Recent advances in eco-friendly and scaling-up bioproduction of prodigiosin and its potential applications in agriculture

TH Nguyen, SL Wang, VB Nguyen - Agronomy, 2022 - mdpi.com
… materials for cost-effective PG production. Overall, low-cost organic sources provide
potentially effective PG biosynthesis and reduce the cost of production with improved PG content …