Recent literature shows the use of chitin and chitosan in electro spinning to produce scaffolds with improved cytocompatibility, which could mimic the native extracellular matrix (ECM). Similarly, silk from the Bombyx mori silkworm, a protein-based natural fibre, having superior machinability, biocompatibility, biodegradation, bioresorbability has evolved as an important candidate for biomedical porous material. The combination of chitin/chitosan and silk fibroin in electrospinning also have potential uses. This review focuses in brief on recent advancements made in chitin, chitosan and silk fibroin based electrospun nanofibrous scaffolds in biomedical fields.