Endothelial differentiation of canine yolk sac cells transduced with VEGF
P Fratini, ACO Carreira, D Alcântara… - Research in Veterinary …, 2016 - Elsevier
Yolk sac (YS) is the site of blood-cell production where primitive erythroid cells originate and
complete their maturation. YS is a source of precursor cells, however its differentiation
potential and suitability for cell therapies are not well described. YS can be a cell source
when neovascularization is required. This study characterized YS canine cells, transduced
with VEGF, to analyze then using Immunocytochemistry, flow cytometry and real time PCR.
Immunocytochemistry: positive expression for CD105, PCNA, VEGF and vWF, flow cytometry …
complete their maturation. YS is a source of precursor cells, however its differentiation
potential and suitability for cell therapies are not well described. YS can be a cell source
when neovascularization is required. This study characterized YS canine cells, transduced
with VEGF, to analyze then using Immunocytochemistry, flow cytometry and real time PCR.
Immunocytochemistry: positive expression for CD105, PCNA, VEGF and vWF, flow cytometry …
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