Endotracheal Aerosolization device for laboratory investigation of pulmonary delivery of nanoparticle suspensions: in vitro and in vivo validation

Z Huang, Y Huang, C Ma, X Ma, X Zhang… - Molecular …, 2018 - ACS Publications
Z Huang, Y Huang, C Ma, X Ma, X Zhang, L Lin, Z Zhao, X Pan, C Wu
Molecular Pharmaceutics, 2018ACS Publications
The objective of this study was to perform the in vitro and in vivo validation of an
endotracheal aerosolization (ETA) device (HRH MAG-4, HM). Solid lipid nanoparticle
suspension (SLNS) formulations with particle sizes of approximately 120, 240, 360, and 480
nm were selected as model nanoparticle suspensions for the validation. The emission rate
(ER) of the in vitro aerosolization and the influence of aerosolization on the physicochemical
properties were investigated. A high ER of up to 90% was obtained, and no significant …
The objective of this study was to perform the in vitro and in vivo validation of an endotracheal aerosolization (ETA) device (HRH MAG-4, HM). Solid lipid nanoparticle suspension (SLNS) formulations with particle sizes of approximately 120, 240, 360, and 480 nm were selected as model nanoparticle suspensions for the validation. The emission rate (ER) of the in vitro aerosolization and the influence of aerosolization on the physicochemical properties were investigated. A high ER of up to 90% was obtained, and no significant alterations in physicochemical properties were observed after the aerosolization. The pulmonary deposition of model drug budesonide in Sprague–Dawley rats was determined to be approximately 80%, which was satisfactory for pulmonary delivery. Additionally, a fluorescent probe with aggregation-caused quenching property was encapsulated in SLNS formulations for in vivo bioimaging, after excluding the effect of aerosolization on its fluorescence spectrum. It was verified that SLNS formulations were deposited in the lung region. The results demonstrated the feasibility and reliability of the HM device for ETA in laboratory investigation.
ACS Publications
以上显示的是最相近的搜索结果。 查看全部搜索结果