Enzymes isolated from thermophilic organisms found in oil reservoirs can find applications
in many fields, including the oleochemical, pharmaceutical, bioenergy, and food/dairy
industries. In this study, in silico identification and recombinant production of an esterase
from the extremophile bacteria Petrotoga mobilis (designated PmEst) were performed. Then
biochemical, bioinformatics and structural characterizations were undertaken using a
combination of synchrotron radiation circular dichroism (SRCD) and fluorescence …