Epigenetic obstacles encountered by transcription factors: reprogramming against all odds
CA Gifford, A Meissner - Current opinion in genetics & development, 2012 - Elsevier
Current opinion in genetics & development, 2012•Elsevier
Reprogramming of a somatic nucleus to an induced pluripotent state can be achieved in
vitro through ectopic expression of Oct4 (Pou5f1), Sox2, Klf4 and c-Myc. While the ability of
these factors to regulate transcription in a pluripotent context has been studied extensively,
their ability to interact with and remodel a somatic genome remains underexplored. Several
recent studies have begun to provide mechanistic insights that will eventually lead to a more
rational design and improved understanding of nuclear reprogramming.
vitro through ectopic expression of Oct4 (Pou5f1), Sox2, Klf4 and c-Myc. While the ability of
these factors to regulate transcription in a pluripotent context has been studied extensively,
their ability to interact with and remodel a somatic genome remains underexplored. Several
recent studies have begun to provide mechanistic insights that will eventually lead to a more
rational design and improved understanding of nuclear reprogramming.
Reprogramming of a somatic nucleus to an induced pluripotent state can be achieved in vitro through ectopic expression of Oct4 (Pou5f1), Sox2, Klf4 and c-Myc. While the ability of these factors to regulate transcription in a pluripotent context has been studied extensively, their ability to interact with and remodel a somatic genome remains underexplored. Several recent studies have begun to provide mechanistic insights that will eventually lead to a more rational design and improved understanding of nuclear reprogramming.
Elsevier