This study aims to evaluate the antioxidant and anticancer activity of secondary metabolite compounds from Myristica fragrans Houtt.(nutmeg) bark using n-hexane extract based on DPPH radical scavenging and microculture tetrazolium salt (MTT) assay. The chemical structural analysis using NMR, FTIR, and LC-MS spectroscopy confirmed and identified the structure of isolated compound namely (2E)-5 (2z. 4E)-hexa-2, 4,-dio-zyl)-2propylcyclohexanol (C 18 H 30 O 4) for the first time which is corresponding for the excellent antioxidant and anticancer activity against MCF-7 cell lines with the IC 50 value of 99.76 and 10.75 ppm, respectively.