The soybean cyst nematode (Heterodera glycine Ichinohe, SCN) is one of the most serious yields limiting pathogen on soybean (Glycine max (L.) Merr) which is found in many soybean–growing regions throughout the world. Applying of resistant sources is one of the efficient measures to manage this nematode. In this study, reaction of 63 soybean lines to the soybean cyst nematode were evaluated in field and growth chamber conditions. Host resistance was determined on the basis of female index (ie, number of female developed on the tested lines/number of female on the standard susceptible cultivar Lee 74 as percentage). The results demonstrated that five lines were defined as resistant, whereas 14 lines with FI 10 to 29 were considered as moderate resistance. Subsequently, 22 lines were demonstrated as moderately susceptible and 22 lines were characterized as susceptible lines. The lines which showed resistance and moderate resistance in field conditions were selected for assay under control conditions. The results of pot experiments conducted under control conditions verified the field findings.